Composite

Part:BBa_K3805329

Designed by: Andi Zhai   Group: iGEM21_BNU-China   (2021-10-21)


Fusion protein with proinsulin and araC

We combined three kinds of proinsulin Normal (BBa_K3805429), Mutation Ⅰ (BBa_K3805666), Mutation Ⅱ (BBa_K3805001) with araC (BBa_I0500), letting proinsulin sequence affect the expression of araC. Here we take proinsulin(Normal) as an example.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1487
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1426
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1261
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1243


[edit]
Categories
Parameters
None